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Most pathways overlapped between 3R4F- and pMRTP-exposed groups, but GSEA identified nine, 13, and two unique pathways for pMRTP in the RNE, bronchi, and parenchyma, respectively (Supplemental Table 11).
An overall heatmap (Supplemental Fig. 4) and a detailed description of GSEA results are provided in Supplemental Results
A detailed analysis of unique pMRTP-related pathways revealed that most *leading edge genes* were also present in the commonly activated pathways with only minor differences in their gene combination.
Because of this high overlap, it is difficult to evaluate why a certain pathway is addressed in a certain tissue.
However, it is conceivable that the addressed pathways, as annotated in these databases, are in an inappropriate tissue context because none are from normal respiratory tract tissues.