Glossary entry (derived from question below)
Spanish term or phrase:
Bacterias: siembra de 10 ml de la dilución 1/10
English translation:
Bacteria: inoculation with 10 mL from the 1:10 dilution
- The asker opted for community grading. The question was closed on 2016-03-13 08:54:08 based on peer agreement (or, if there were too few peer comments, asker preference.)
Mar 9, 2016 22:27
8 yrs ago
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Spanish term
Spain
A team Engineer + Lawyer + Biochemist
Native in: Spanish 
, English 
Works in: English to Spanish, Spanish to English, Spanish to French, and 5 more.
Bacterias: siembra de 10 ml de la dilución 1/10
Spanish to English
Medical
Biology (-tech,-chem,micro-)
microbiología
yo he traducido al inglés:
Bacteria: Inoculating of 10 mL of the 1/10 dilution
Ruego me expliquéis si lo he hecho bien. He mirado en Google y también en el IATE . Pero no soy bióogo y tengo duda. Para ayudar pongo mas contexto: * Bacterias: siembra de 10 ml de la dilución 1/10 por filtración en 2 placas de agar TSA seguido de incubación a 30-35ºC durante 5 días.
* Hongos: siembra de 10 ml de la dilución 1/10 por filtración en 2 placas de agar TSA seguido de incubación a 30-35ºC durante 5 días.
Bacteria: Inoculating of 10 mL of the 1/10 dilution
Ruego me expliquéis si lo he hecho bien. He mirado en Google y también en el IATE . Pero no soy bióogo y tengo duda. Para ayudar pongo mas contexto: * Bacterias: siembra de 10 ml de la dilución 1/10 por filtración en 2 placas de agar TSA seguido de incubación a 30-35ºC durante 5 días.
* Hongos: siembra de 10 ml de la dilución 1/10 por filtración en 2 placas de agar TSA seguido de incubación a 30-35ºC durante 5 días.
Proposed translations
3 hrs
Muriel Vasconcellos
United States
United States
Native in English
Works in field
United States
Each translation a gem!
Native in: English (Variant: US)
Works in: Spanish to English, Portuguese to English
Related KudoZ points
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43
Selected
Bacteria: inocuation of 10 mL of/from the 1:10 dilution
IMO, you were correct to begin with.
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Note added at 4 hrs (2016-03-10 03:06:26 GMT)
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Here is an excerpt about the spread plate technique:
http://www.microbelibrary.org/component/resource/laboratory-...
One method of distributing bacteria evenly over the surface of an agar plate medium is commonly referred to as the spread plate method. Classically a small volume of a bacterial suspension is spread evenly over the agar surface using a sterile bent glass rod as the spreading device. The goal in evenly distributing the bacterial suspension is typically to permit the growth of colonies that can subsequently be enumerated (see Serial Dilution Protocols) and/or sampled following incubation. Each plate is spread with a single inoculum of the bacterial suspension.
An alternative approach to spreading a single inoculum volume with a smooth device is to apply a smaller volume and tip the plate, allowing gravity to distribute the inoculum in a band or track (track method) or to allow the inoculum to dry in place (drop method). With this alternative approach, several sample dilutions can be distributed on a single agar plate.
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Note added at 4 hrs (2016-03-10 03:06:26 GMT)
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Here is an excerpt about the spread plate technique:
http://www.microbelibrary.org/component/resource/laboratory-...
One method of distributing bacteria evenly over the surface of an agar plate medium is commonly referred to as the spread plate method. Classically a small volume of a bacterial suspension is spread evenly over the agar surface using a sterile bent glass rod as the spreading device. The goal in evenly distributing the bacterial suspension is typically to permit the growth of colonies that can subsequently be enumerated (see Serial Dilution Protocols) and/or sampled following incubation. Each plate is spread with a single inoculum of the bacterial suspension.
An alternative approach to spreading a single inoculum volume with a smooth device is to apply a smaller volume and tip the plate, allowing gravity to distribute the inoculum in a band or track (track method) or to allow the inoculum to dry in place (drop method). With this alternative approach, several sample dilutions can be distributed on a single agar plate.
4 KudoZ points awarded for this answer.
Comment: "Thank you. for helping me in this term. I appreciate your help very much LuisLarra"
18 mins
Bacteria: spread plate 10 ml of the 1:10 dilution
Volume to Volume - Wellesley College
academics.wellesley.edu/Biology/Concepts/Html/volumetovolume.html
25 Aug 2004 - For example, to make a 1:10 dilution of a 1M NaCl solution, you would ... For example: if you needed 10 mL of the 1:10 dilution, then you would ...
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Note added at 21 mins (2016-03-09 22:48:20 GMT)
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Genetic Transformation Systems in Fungi
https://books.google.co.uk/books?isbn=3319105035
Marco A. van den Berg, Karunakaran Maruthachalam - 2014 - Science
a10 μg of DNA in X μL (plus DNA) or X μL of water (minus DNA; as a control) ... glass tubes (about 15 mL in each) and dilute with equal volume of 1×STC. ... (d) 100 μL+900 μL of 1 ×STC; spread 100 μL of this 1:10 diluted sample (S1) on ...
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Note added at 21 mins (2016-03-09 22:48:56 GMT)
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or just
Bacteria: spread 10 ml of the 1:10 dilution
academics.wellesley.edu/Biology/Concepts/Html/volumetovolume.html
25 Aug 2004 - For example, to make a 1:10 dilution of a 1M NaCl solution, you would ... For example: if you needed 10 mL of the 1:10 dilution, then you would ...
--------------------------------------------------
Note added at 21 mins (2016-03-09 22:48:20 GMT)
--------------------------------------------------
Genetic Transformation Systems in Fungi
https://books.google.co.uk/books?isbn=3319105035
Marco A. van den Berg, Karunakaran Maruthachalam - 2014 - Science
a10 μg of DNA in X μL (plus DNA) or X μL of water (minus DNA; as a control) ... glass tubes (about 15 mL in each) and dilute with equal volume of 1×STC. ... (d) 100 μL+900 μL of 1 ×STC; spread 100 μL of this 1:10 diluted sample (S1) on ...
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Note added at 21 mins (2016-03-09 22:48:56 GMT)
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or just
Bacteria: spread 10 ml of the 1:10 dilution
2 hrs
Robert Carter
Mexico
Mexico
Native in English
Mexico
Translating full-time since 1998
Native in: English (Variant: British)
Works in: Spanish to English
Bacteria: inoculation from 10 ml of the 1:10 dilution
I think inoculation is perfectly fine here, and I imagine it's done from 10 ml rather than with, since using the whole 10 ml would be quite a lot for two agar plates.
Bacteria may be introduced to the media (inoculated) by various means. Usually the bacteria e.g. from a drop in a heat-sterilised loop are spread on the surface of (ready set) agar.
http://www.biotopics.co.uk/microbes/tech1.html
Un microorganismo se puede sembrar en un medio líquido o en la superficie de un medio sólido de agar.
https://es.wikipedia.org/wiki/Cultivo_(microbiología)
Once the growth medium in the petri dish is inoculated with the desired bacteria, the plates are incubated at the best temperature for the growing of the selected bacteria (for example, usually at 37 degrees Celsius for cultures from humans or animals, or lower for environmental cultures).
https://en.wikipedia.org/wiki/Microbiological_culture
Bacteria may be introduced to the media (inoculated) by various means. Usually the bacteria e.g. from a drop in a heat-sterilised loop are spread on the surface of (ready set) agar.
http://www.biotopics.co.uk/microbes/tech1.html
Un microorganismo se puede sembrar en un medio líquido o en la superficie de un medio sólido de agar.
https://es.wikipedia.org/wiki/Cultivo_(microbiología)
Once the growth medium in the petri dish is inoculated with the desired bacteria, the plates are incubated at the best temperature for the growing of the selected bacteria (for example, usually at 37 degrees Celsius for cultures from humans or animals, or lower for environmental cultures).
https://en.wikipedia.org/wiki/Microbiological_culture
Peer comment(s):
| neutral |
Muriel Vasconcellos
: You make a good point. The text doesn't say what the dilution is. It could be dilution of the agar itself. The inoculum is usually a much smaller amount. But the syntax is telling me that 10 mL is the inoculum.
1 hr
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What makes you to think that Muriel? 5 ml per agar plate seems a lot to me, am I off-base here?//Okay, I don't agree about the syntax, but thanks anyway for giving me your rationale. Saludos.
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Discussion
http://www.microbelibrary.org/component/resource/laboratory-...
(I have posted the pertinent passage in my answer - it doesn't fit here.)
Spread Plate
www2.hendrix.edu/biology/CellWeb/Techniques/microspread.html
Quantifying Bacteria by Spread Plate. The number of bacteria in solution can be readily quantified by using the spread plate technique. In this technique, the ...